Ventricular fibrillation (VF) in guinea pig heart. VF was induced by burst stimulation (35 ms interval, 100 pulses) and field of view is exactly 1x1 cm (1:1 magnification) from anterior region of heart. The first derivative of action potential was calculated to detect wave fronts as shown in this trace. The animation of action potential propagation clearly shows details of the direction and the curvature of the wave front. The heart was retrogradely perfused at 70 mmHg perfusion pressure and 20 ul of di-4 ANEPPS stock solution (1 mg / 1 mL DMSO).
- Provided by
- Dr.Bum-Rak Choi, Cardiovascular Research Center, Rhode Island Hospital and Brown Medical School