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MiCAM02 was discontinued. Next model MiCAM03 will be released in 2017.

Cardiac Imaging MiCAM ULTIMA

Overview

MiCAM02 is able to achieve optimal S/N ratios among CCD/CMOS cameras. It can be used effectively for the high speed imaging of cardiac activity including isolated hearts and cardiac myocytes in a monolayer. The following applications are common uses for the MiCAM02: intrinsic signal imaging, calcium ion imaging, membrane potential imaging, time-lapse imaging, and general-high speed-imaging.

Description

MiCAM02 has been developed as the next generation of MiCAM01 by Brainvision Inc. MiCAM01 has already achieved a high level of success among high speed imaging systems. MiCAM02 maintains the basic concepts of MiCAM01 such as high speed and high S/N ratios, but it has been significantly improved with regards to spatial resolution, sensitivity, and dark noise/read-out noise levels. Additional software functions including the ability to do time-lapse recordings, have also been implemented.

MiCAM02 can image at speeds up to 1.7 kHz (MiCAM02-CMOS), with 75% real quantum efficiency (MiCAM02-HR), and over 68dB dynamic range (MiCAM02-CMOS). These cameras can be used for both of calcium ion and membrane voltage imaging applications.


Features

CMOS camera and CCD camera are available
Maximum frame rate is 1 kHz

The optical mapping of membrane potential in cultured myocytes or isolated heart tissue is generally easier than imaging neural activity, because the signals are larger and the biological samples tend to be more robust. Two types of cameras are available with the MiCAM02 system, a CMOS camera for high speed imaging, or a 1/2" CCD camera for imaging larger areas at slightly higher S/N ratios.

Spatial resolution is adjustable according to frame rate

Spatial resolution of MiCAM02 is varied at 40x28 - 376x252 pixels according to the frame rate selected. This flexibility with regards to binning provides higher spatial and temporal resolution.

Powerful in dark light levels

The new "H-bin mode" function (only for MiCAM02-HR and HS) reduces dark noise, and it is comparable to that of a cooled-CCD camera. This function is very effective for situations of dark light or fluorescence.

Synchronized Dual-camera system for dual-wave length imaging

MiCAM02 processor has two camera ports, and can be adapted as a dual-camera system using an optional second camera, which provides synchronized recordings. The dual-camera system can be used for the ratio-imaging with voltage-sensitive dyes or calcium ion indicators, as well as imaging with multiple probes. Another advantage of the dual camera system is the ability to image the isolated heart in a 3D perspective.


Multiple signal inputs and outputs

Multiple signal inputs and outputs, which are generally required for research equipment, are also available. Stimulus pulse outputs with arbitrary frequency are implemented, as well as the ability to synchronize recordings of signals from external equipment. These functions allow for faster and easier image data analysis.

User-friendly software

Data analysis software "BV_Ana," which has many useful functions, also includes acquisition capabilities resulting in simpler, smoother, and faster experiments. The quick analysis ability of recorded data makes it possible for users to do many experiments on a single biological sample under various conditions.

Applications

  • Measuring changes in membrane potential by using voltage-sensitive dye such as Di-4-ANEPPS
  • Calcium imaging at higher speed
  • FRET
  • Intrinsic Imaging Based on Hemoglobin and Flavoprotein
  • Fluorescence ratiometric imaging with dual camera system
  • Detection of small changes in light intensity at high speeds
  • Imaging of propagations in brain slices, tissue block, and in vivo samples

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Cardiac Imaging