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Neuronal Imaging BV-XB1

Overview

BV-XB1 was developed in response to requests from labs saying that "MiCAM high speed is not always necessary, but higher spatial resolution is desired.” BV-XB1 is a high resolution imaging system which combines a high resolution camera with 1920 x 1200 pixels, plus image acquisition software developed for bioimaging experiments.

The system includes ESTM-9, which is equipped with external camera control, external illumination control, pulse outputs, analog outputs, and analog inputs recording functionality. By connecting external equipment such as a light source, electrical stimulator, and amplifiers, ESTM-9 synchronizes image acquisition with inputs/outputs of external equipment.

In combination with Brainvision’s fluorescence macroscope, it is possible to construct a wide-field / high resolution / high S/N ratio fluorescence imaging system.

 

Features of XB1CM Camera

XB1CM camera

In the past, it was thought that that CCD sensors are superior under low light condition. In the past several years, however, CMOS sensors suitable for low light condition have been developed due to recent improvements of CMOS technology.

The dark noise of the sensor used in the XB1CM camera head is as low as that of CCD sensors, and it is possible to capture images at relatively high frame rates. Due to this combination of low noise and relatively high speed, XB1CM is suitable for low light level bioimaging applications, such as calcium imaging, imaging using fluorescent proteins such as GCaMP/FRET, and flavoprotein intrinsic imaging.

High resolution and large sensor

Spatial resolution: 1920x1200 pixels, sensor size:11.3mmx7.1mm


Wide dynamic range and low dark noise

A large well capacity of about 32ke- results in greater dynamic range than CCD sensors, and low dark noise comparable to a CCD camera. It can be used for wide range of applications from low to medium light conditions.


Maximum frame rate and sensitivity can be changed by number of pixels and binning


Selected number of pixels Binning Number of active pixels
(HxV)
Maximum frame rate
(fps)
Full pixel
readout(*1)
1920x1200 1x1 1920x1200 78.11
2x2 960x600 128.77
3x3 640x400 128.77
4x4 480x300 128.77
Partial readout
(*2)
960x600 1x1 960x600 249.44
4x4 240x150 249.44
768x480 4x4 192x120 307.03
640x400 1x1 640x400 362.82
4x4 160x100 362.84
480x300 1x1 480x300 469.48
384x240 1x1 384x240 570.13

(*1) Size of effective pixel will change, dependent on binning.
(*2) Size of effective sensor area and size of effective pixel will change, depending on selected number of pixels and binning.

 

Features of ESTM-9

By connecting external equipment such as camera, light source, electrical stimulator, and amplifiers, ESTM-9 can synchronize image acquisition with inputs/outputs of external equipment. ESTM-9


Acquisition Control of External Camera

  • Outputs frame timing pulses to external camera, or receives frame timing signals from external camera.
  • Frame rate can be input.
  • Time-lapse acquisition control is also available.
Acquisition Control


Illumination Control for External Light Source

  • Outputs signals to external light source in synchronization with camera control.
  • Continuous, intermittent, or custom light pattern available.

Pulse Stimulator

  • 4 channels of pulse stimulation can be controlled individually.
  • Stimulation works with or without camera control.
  • Continuous stimulation using free-run mode is also available.
Pulse Stimulator


Analog Output - Custom Pulse Pattern

  • 2 channels of analog outputs.
  • Pulses 1-4 can be generated and output from Aout 1.
  • Sine, triangle, sawtooth, or custom waveform can be selected.
  • Custom pulse pattern can be created using a CSV file.
Analog Output

Analog Output

Analog Output


Analog Signal Recording

  • 2 channels of analog inputs.
  • Pre-trigger, delayed-trigger, and multiple trigger source available.
Analog Signal Recording Analog Signal Recording

 

Other Features

Turnkey system ready for immediate use

The system consists of hardware/software specifically designed for high-speed fluorescence imaging, and the PC and monitor are included as standard equipment. Before use, calibration/setting/tuning is not necessary. The hardware connection is straightforward between components using cables, making it possible to acquire data immediately after setup.

Diagram

Multi camera system

By connecting multiple XB1CMs to PC, it is possible to operate as a fully synchronized multi camera system. Two images can be superimposed on monitor display so that user can overlap two images.


Time lapse imaging

BV-XB1 can capture phenomenon that changes in a few minutes up to several hours, intermittently. Because illumination light is turned on only at the time of image acquisition, phototoxic effects to sample are minimized, and a stable baseline can be acquired.
Time LapseExample of time-lapse imaging

 

Main Features of Software

Acquisition Software (BV Workbench)

BV Workbench is the image acquisition software that controls MiCAM03 imaging system. In addition to image acquisition and control of inputs and outputs of the imaging system, analysis functions including movie playback, wave display, filter functions, etc…are also included. BV Workbench has an intuitive user interface that is easily operated with the use of a mouse.

BV Workbench    BV WorkbenchAcquisition Setting                                           Monitor windows and wave display

BV Workbench    BV WorkbenchData analysis                                                     2 image overlay


 

Features
  • Synchronized control of multiple imaging systems and electrical stimulator
  • Image acquisition mode: Single acquisition (Save to PC memory or direct recording to SSD/HDD), Averaging, Time Lapse Imaging
  • Image overlay, Image Flip/Rotation
  • Wave display of light intensity change of pixel (real-time or after image acquisition)
  • Light intensity histogram
  • Filtering (Gaussian, Mean, Median, Inverse polarity)
  • Tools (alignment correction、illumination correction, binning, crop, time range)
  • Graphic Diagram of simulation pulse pattern

 

Analysis Software (BV_Ana)


BV_Ana

BV_Ana is the software used to analyze optical imaging data. It was developed mainly for analysis of neuronal activity and cardiac activity using voltage sensitive dyes.

By superimposing the change in light intensity of each pixel onto the background image, the location of light intensity changes as well as the percentage of light intensity change can be easily analyzed. It is also very straightforward to create presentation materials in the form of images and movies.

Tile Image DisplayTime Differential Processing4 image simultaneous display Tile Image Display                  Time Differential Processing                      4 image simultaneous display

Isochronous mapsIsochronous mapsMulti Wave Display Isochronous maps    Isochronous maps    Multi Wave Display


 

Features
  • Innovative software capable of intuitive operations
  • Bleaching/Drift curve correction functions
  • Enhanced processing functions such as high-pass/low-pass filters, fast fourier transform function
  • Ability to import 16bit gray-scale multi-TIFF format data from other imaging systems
  • Export data such as images and movies, which can be used as presentation materials

Specifications
Image Display Import data from cameras having up to 512 x 512 pixels, simultaneous display of up to 4 images per data, waveform display of up to 20 x 20 area, time image display, dF display, absolute intensity display (actual image display), gain/offset/threshold adjustments, color bar display, color reversal, black and white display, etc.
Time control Specify frame number, specify time range on waveform, movie playback, movie speed control, frame-by-frame playback, display frame number or time, specify arbitrary frame at zero time point, time stamp to arbitrary frame
Wave display Display waveform by double-clicking on image, specify range of displayed frames, display setting for time, scaling/fixing of displayed time range, specify x/y axis scale, F value/dF value/amount of percentage change display, gain setting, spatial filter, temporal filter, time line display, light intensity change display, data processing using zero/soft cubic/circle filters
Import Data format of MiCAM05, MiCAM03, MiCAM ULTIMA, MiCMA02, MiCAM01, HNI-1250, Fuji HR Deltaron and 16bit gray-scale multi-TIFF format data
Export AVI/BMP/TIFF output of image only, AVI output of selected range of analysis area, BMP/JPEG/ASCII/ binary output of analyzed data, CSV output of image and waveform
System requirements OS: Windows 7/8/10, RAM: 256MB or more Monitor: 1,920x1,200 recommended (or 1,280x1,024)

 

Applications

  • Calcium imaging
  • Intrinsic fluorescence imaging based on flavoproteins and hemoglobin
  • Imaging FRET, GCaMP, and various fluorescent proteins such as VSFP

 

Specifications

System
Name High Resolution Imaging System BV-XB1
Model BV-XB1
Standard System
Configuration
XB1 camera head (XB1CM), ESTM-9,
Cables, PC, Monitor
Acquisition software, Analysis Software
Supported OS Windows 7/10 64bit
Camera Head
Name XB1 Camera Head
Model XB1CM
Image Sensor SONY IMX174 CMOS (1/1.2inch)
Resolution (H x V) 1920 x 1200
Active Image Area (H x V) 11.3mm x 7.1mm
Pixel Size (H x V) 5.86um x 5.86um
Shutter Mode Global Shutter
Maximum Frame Rate
and Pixels
78.11fps@1920x1200pixels
249.44fps@960x600pixels
362.82fps@640x400pixels
469.48fps@480x300pixels
570.13fps@384x240pixels
Quantum Efficiency 70%
Dark Noise 6.6e-
Saturation Capacity 31.7ke-
Dynamic Range 73.7dB
Pixel Bit Depth 12bit
Interface USB3.0
Lens Mount C-mount
Dimension 29.3mm x 29mm x 29mm
Weight 80g
ESTM-9
Pulse Output 4 ch (BNC)
Analog Input 2 ch (BNC)
Analog Output 2 ch (BNC)
Camera I/O Port for XB1CM 2 ch (4 pin connector)
Frame Timing Signal Output 1 ch (BNC)
Acquisition Status Output 1 ch (BNC)
Shutter Control Output 1 ch (BNC)
External Trigger Input 1 ch (BNC)
Others Trigger button, Stop button,
8-pin connector for optional function
Control Software 2 versions
Interface USB2.0
Power USB bus power
Includes ESTM-9, USB2.0 cable, Software
Dimensions 180mm x 120mm x 65mm
Each function
Main Trigger Trigger Mode Software / External / Internal
Trigger Source Software / External Trigger Input / Trigger Button
Trigger Edge Positive / Negative
Pulse Output Output Voltage 5V
Clock Cycle 10μsec - 10msec
Duration time 10μsec - 10min
Delay Time 10μsec - 10min
Interval Time 10μsec - 10min
Pulse Number 1 - 65,535
Analog Input Input Range 1V / 3V / 10V
Sample Clock 10μsec - 10msec
Trigger Source Main / Pulse1 / Pulse2 / Pulse3 / Pulse4 / Ain1 level
Others Previous-trigger, Delayed-trigger,
Low-pass filter, Polarity inversion
Analog
Output
Output Voltage -10V - 10V
Output Waveform Sine, Triangle, Sawtooth,
Custom (with a CSV file)
Camera Control Number of Camera Port Brainvision BV-XB1 : 2 (I/O connectors)
Other camera : 1 (BNC connectors)
Frame Timing Signal Output or Input
Frame Timing Signal Cycle 10μsec-300msec
Trigger Source Main/Ain1 level/Pulse3
Others Time lapse
Light Control Lighting Mode Continuous
Synchronous with data acquisition /
Synchronous with frame acquisition /
Custom lighting pattern
Others Polarity inversion

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