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Neuronal Imaging Sample Data

Mouse In Vivo Imaging using Voltage-Sensitive Dye (MiCAM ULTIMA)

Mouse In Vivo Imaging using Voltage-Sensitive Dye


Voltage-sensitive dye imaging of in-vivo mouse with MiCAM ULTIMA.
This data was obtained from a 2 month-old mouse that was under urethane anaesthesia (1.75 g/kg). A heating pad was used to maintain the measured body temperature of the mouse at 37°C. Two electrodes were inserted under the skin of the forearms to receive the electrocardiogram signal which was used to trigger the MiCAM ULTIMA acquisition.

Animal
Mouse
Sample
In Vivo (Somatosensory Barrel Cortex)
Method
Piezo Whisker Flick Stimulation
Dye
RH1691
Sampling Rate
2.0ms/frame
Averaging
10 times
Provided by
Dr.Isabelle Ferezou and Dr.Carl C.H. Petersen
Brain Mind Institute, EPFL, Switzerland
Reference
Visualizing the Cortical Representation of Whisker Touch: Voltage-Sensitive Dye Imaging in Freely Moving Mice.
Neuron. 2006 May 18;50(4):617-29.

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Neuronal Imaging