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  3. MiCAM03: Overview

Specifications of MiCAM05-N256 and MiCAM03-N256

Model MiCAM05-N256 MiCAM03-N256
Camera Head
Image Sensor Original CMOS
Active Pixels (H x V) 256 x 256
Active Image Area (H x V) 17.6mm x 17.6mm
Pixels Size (H x V) 69μm x 69μm
Shutter Mode Global Shutter
Quantum Efficiency 55%@550nm / 50%@600nm
Well-Depth (Dark Noise) 3,000,000 e- (500 e-) / 600,000 e- (130 e-)
Dynamic Range 71dB
A/D Converter 14bit
Lens Mount M42 mount (Option: C or F mount)
Processor
Camera Interface 1 (up to 2) built-in
Camera Port 2 (up to 4) 2
Buffer Memory
Inside Processor
4GB (up to 8GB) 1GB
Analog Inputs for
Synchronous Recording
4ch 2ch
Output for Stimulation Pulse 2ch 1ch
Input for
External Trigger Signal
1ch 1ch
Output for Lighting Control 1ch 1ch
Input/Output for Frame Timing 1ch 1ch
Output for Real Time
Light Intensity Monitor
8ch -
Output for
Light Intensity Control
2ch -
Current Output 2ch -
Output for Acquisition Status 1ch -
Output for Trigger Wait Status 1ch -
Functions
1. Maximum Frame Rate and
Pixels (for 1 camera)
1,923fps@256x256pixels
3,125fps@192x192pixels
5,556fps@128x128pixels
20,000fps@ 32x 32pixels
1,000fps@256x256pixels
1,000fps@128x128pixels
2. Single Trial Acquisition
3. Long Time Acquisition
(direct saving to disk)
(Optional)
4. Averaging Acquisition
5. Multiple ROI Readout for
Higher Frame Rate
(Optional)
6. Time-Lapse Acquisition (Optional)
7. High Resolution Camera
Control
(Optional) (Optional)
Software
8. Acquisition Software
(BV Workbench)
9. Analysis Software (BV_Ana) (Optional)

 

1. Maximum Frame Rate and Pixels

 

Table1: Selectable Number of Pixels and Maximum Frame Rate
(1 camera, noise reduction mode=2 times averaging)
Selectable Number of Pixels (HxV) Maximum Frame Rate (fps)
    MiCAM05-N256         MiCAM03-N256    
256 x 256 Full Resolution 1,923 1,000
192 x 192 Square ROI 3,125 -
128 x 128 5,556 1,000
64 x 64 12,500 (The same setting as
MiCAM05-N256
can be used by option)
256 x 128 Horizontally Long
Rectangle ROI
3,448
256 x 64 5,555
256 x 32 8,333
128 x 256 Vertically Long
Rectangle ROI
3,333
64 x 256 5,000
32 x 256 7,143
32 x 32 16 blocks 2,632
15 blocks 2,778
14 blocks 2,941
13 blocks 3,125
12 blocks 3,448
11 blocks 3,704
10 blocks 4,000
 9 blocks 4,348
 8 blocks 5,000
 7 blocks 5,556
 6 blocks 6,250
 5 blocks 7,143
 4 blocks 8,333
 3 blocks 11,111
 2 blocks 14,286
 1 blocks 20,000

 

2. Single Trial Acquisition


Single trial. Acquired data is temporarily stored in RAM of PC. Data can be saved to PC after acquisition finishes. Because PC’s RAM is used as the buffer memory for acquired data, the total number of recordable frames is less than the total number or recordable frames in [3. Long Time Acquisition] mode.

 

3. Long Time Acquisition (MiCAM03: option)


SSD

Single trial. Acquired data is automatically saved to drive of PC during data acquisition. Because acquired data is written directly to SSD (Solid State Disk) or HDD, it is possible to record for a longer time than with [3. Single Trial Acquisition] mode.

Maximum recordable time depends on frame rate, number of pixels, number of cameras, CPU usage rate etc. Please refer to the PDF file for the approximate number of recordable frames and recordable time (MiCAM03 options are highlighted in yellow).

 

4. Averaging Acquisition


Averaging trials. Acquired data is temporarily stored in RAM of PC. Data can be saved to PC after acquisition finishes. Each trial data can be automatically saved, in addition to averaged data. When noise occurs during acquisition, it is also possible to perform offline averaging while excluding noisy data.

 

5. Multiple ROI Readout for Higher Frame Rates (MiCAM03: option)


Preset partial readout setting and multiple ROI setting can be selected, and as a result it is possible to achieve higher frame rates. For example, ultra high frame rates such 20,000fps@32x32 pixels is possible.

In the standard model of MiCAM03, only 256x256 pixels and 128x128 pixels can be selected, and the maximum frame rate is 1,000fps. By selecting this ROI option, higher frame rates such as 1,923fps@256x256 pixels, and 5,556fps@128x128 pixels, is available. In addition, multiple ROI readout can be also used. Please refer to Table 1.

         All pixels readout (256x256 pixels)         multiple ROI readout (32x32 pixels x 9 area)


 

6. Time-Lapse Acquisition (MiCAM03: option)


MiCAM03 can capture phenomenon that changes in a few minutes up to several hours, intermittently. Because illumination light is turned on only at the time of image acquisition, phototoxic effects to sample is minimized, and a stable baseline can be acquired.

Time LapseExample of Time-lapse imaging


 

7. High Resolution Camera Control (MiCAM05/MiCAM03: option)


It is possible to control a high resolution camera having 1920x1200 pixels (BV-XB1 camera). High resolution imaging can be applied to various applications, including detection of intracellular Ca 2+ dynamics, flavoprotein fluorescence, FRET imaging, and also imaging of animal behaviors. High resolution imaging with BV-XB1 can also be synchronized with MiCAM03 high-speed imaging.

BV-XB1Example of flavoprotein imagingBV-XB1                                    Example of flavoprotein imaging


 

8. Acquisition Software BV Workbench


BV Workbench is the image acquisition software that controls MiCAM03 imaging system. In addition to image acquisition and control of inputs and outputs of the imaging system, analysis functions including movie playback, wave display, filter functions, etc…are also included. BV Workbench has an intuitive user interface that is easily operated with the use of a mouse.

BV Workbench    BV WorkbenchAcquisition Setting                                           Monitor windows and wave display

BV Workbench    BV WorkbenchData analysis                                                     2 image overlay



Features
  • Synchronized control of multiple imaging systems and electrical stimulator
  • Image acquisition mode: Single acquisition (Save to PC memory or direct recording to SSD/HDD), Averaging, Time Lapse Imaging
  • Image overlay, Image Flip/Rotation
  • Wave display of light intensity change of pixel (real-time or after image acquisition)
  • Light intensity histogram
  • Filtering (Gaussian, Mean, Median, Inverse polarity)
  • Tools (alignment correction、illumination correction, binning, crop, time range)
  • Graphic Diagram of simulation pulse pattern

 

9. Analysis Software BV_Ana (MiCAM03: option)


BV_Ana

BV_Ana is the software used to analyze optical imaging data. It was developed mainly for analysis of neuronal activity and cardiac activity using voltage sensitive dyes.

By superimposing the change in light intensity of each pixel onto the background image, the location of light intensity changes as well as the percentage of light intensity change can be easily analyzed. It is also very straightforward to create presentation materials in the form of images and movies.

Tile Image DisplayTime Differential Processing4 image simultaneous display Tile Image Display                  Time Differential Processing                      4 image simultaneous display

Isochronous mapsIsochronous mapsMulti Wave Display Isochronous maps    Isochronous maps    Multi Wave Display



Features
  • Innovative software capable of intuitive operations
  • Bleaching/Drift curve correction functions
  • Enhanced processing functions such as high-pass/low-pass filters, fast fourier transform function
  • Ability to import 16bit gray-scale multi-TIFF format data from other imaging systems
  • Export data such as images and movies, which can be used as presentation materials

Specifications
Image Display Import data from cameras having up to 512 x 512 pixels, simultaneous display of up to 4 images per data, waveform display of up to 20 x 20 area, time image display, dF display, absolute intensity display (actual image display), gain/offset/threshold adjustments, color bar display, color reversal, black and white display, etc.
Time control Specify frame number, specify time range on waveform, movie playback, movie speed control, frame-by-frame playback, display frame number or time, specify arbitrary frame at zero time point, time stamp to arbitrary frame
Wave display Display waveform by double-clicking on image, specify range of displayed frames, display setting for time, scaling/fixing of displayed time range, specify x/y axis scale, F value/dF value/amount of percentage change display, gain setting, spatial filter, temporal filter, time line display, light intensity change display, data processing using zero/soft cubic/circle filters
Import Data format of MiCAM05, MiCAM03, MiCAM ULTIMA, MiCMA02, MiCAM01, HNI-1250, Fuji HR Deltaron and 16bit gray-scale multi-TIFF format data
Export AVI/BMP/TIFF output of image only, AVI output of selected range of analysis area, BMP/JPEG/ASCII/ binary output of analyzed data, CSV output of image and waveform
System requirements OS: Windows 7/8/10, RAM: 256MB or more Monitor: 1,920x1,200 recommended (or 1,280x1,024)

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