1. Top
  2. Neuronal Imaging
  3. MiCAM ULTIMA: Overview

MiCAM ULTIMA was discontinued. Next model MiCAM05 is now released.

Neuronal Imaging MiCAM ULTIMA

Overview

MiCAM ULTIMA‚ is a CMOS based camera that optimizes high speeds, high resolution, and high signal to noise ratios. It is designed to detect very small changes in fluorescent light levels of biological samples that are stained with fluorescence probes such as voltage-sensitive dyes or calcium indicators.

Description

MiCAM ULTIMA is the latest CMOS imaging system that has made ideal advances with regards to super high-speed performance and optimal S/N ratios. It is based on previous models of the MiCAM series. This imaging system is the premier high-end model of the MiCAM series developed by BrainVision Inc., which has substantial experience in the design of extremely fast and highly sensitive imaging systems.

The unique and highly customized CMOS sensor has achieved a high-speed image acquisition rate of up to 10,000 frames/second and a quantum efficiency of 60% resulting in a dynamic range of 70db or more. High-speed imaging with voltage-sensitive dyes is one of the most difficult fluorescence imaging applications. However, the ULTIMA CMOS image sensor is easily capable of measuring fluorescence changes with voltage sensitive dyes at optimal S/N ratios.

Features

Maximum Frame Rate of 0.1msec/frame (10KHz)

Action potential propagation
of rat hippocampus slice.
(0.1msec/frame,
acquired with 4 times averaging)

In order to record small changes in fluorescence of biological samples stained with voltage sensitive dyes, the ideal combination of high sensitivity and high speed is required. MiCAM ULTIMA, which overcomes these challenges with BrainVision's cutting-edge research and development, can detect small changes in fluorescence such as 0.1% After acquisitions, users can easily analyze signal propagation between neurons of samples such as hippocampus slices at speeds up to 10,000 frames per second and only 4 times averaging.


Real-Time Imaging of Action Potential Propagation with Single Scan

Temporal resolution of 0.1msec/frame, spatial resolution of 100x100pixel, and dynamic range of 70db makes it possible for scientists to record neuronal activity at speeds of several millisecond/frame even without any averaging acquisition (single scan).


Huge Well Depth of Single Pixel (10Me- / 1.5Me-)

Sufficient fluorescence light intensity is needed to detect small changes in fluorescence with a single sweep. MiCAM ULTIMA, which has a huge well depth (10Me- / 1.5Me-), can achieve high S/N ratios without any pixel saturation in most bright light conditions.


Completely Synchronized Dual Camera System

MiCAM ULTIMA processor has two camera ports. The ULTIMA can operate as a synchronized dual camera system by the simple addition of a slave camera and connection to the existing processor. This allows for dual-wavelength imaging or dual-staining with multiple probes such as voltage-sensitive dyes and calcium indicators.

Software with Intuitive Interface


BV_Analyzer is a data analysis software and is included with all of the MiCAM systems. This software has various functions, such as wave display, 3D display, animation display, real-image display, spatial/temporal filter, high-pass/low-pass filter, AVI/TIFF/BMP/CSV file export, etc.

Characteristics of Wave Spectral Sensitivity

This sensor has a wide range of spectral sensitivity between near-infra red (800-900nm) to ultraviolet (300-400nm), and has a high quantum efficiency of 50-60%. As a result, the ULTIMA is suitable for various imaging techniques with many different kinds of fluorescent dyes.


Applications

  • Measuring changes in membrane potential by using voltage-sensitive dye such as Di-4-ANEPPS
  • Calcium imaging at higher speed
  • FRET
  • Intrinsic Imaging Based on Hemoglobin and Flavoprotein
  • Fluorescence ratiometric imaging with dual camera system
  • Detection of small changes in light intensity at high speeds
  • Imaging of propagations in brain slices, tissue block, and in vivo samples

Page top


Neuronal Imaging