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Fluorescence Beam Splitting System DLFLSP2R

Overview

The DLFLSP2R is a fluorescent beam splitter that transmits two wavelengths of light simultaneously to two cameras. It has been designed for applications such as simultaneous imaging of biological preparations stained with both a calcium indicator and a voltage-sensitive dye, as well as fluorescent ratiometric imaging using calcium indicators or voltage-sensitive dyes.

The DLFLSP2R can be with or without Brainvision's tandem-lens macroscope (see Fig.1 and 2). Both configurations adopt the tandem-lens style which includes Leica PLAN APO lenses, wide optical pathway, and large custom-made fluorescence filter cubes. These systems are ideal for detecting very small signals which are typical in membrane voltage imaging.

The DLFLSP2R splits fluorescent light emitted from a biological preparation into two different wavelengths to two separate cameras, with the use of a large, custom-made dichroic mirror. The dichroic mirror uses a kinetic mount with straightforward shift and tilt adjustment knobs which allows users to easily overlay images of two different wavelengths.

An excitation light source light guide can be connected to the side of the DLFLSP2R with the use of an adaptor (fig.3). In this way, the DLFLSP2R can be used as an epi-fluorescence filter unit for single wavelength fluorescence imaging.


Fig.1 (left) Setting for imaging two wavelengths simultaneously by combining THT macroscope (uses epi-illumination)
Fig.2 (center) Setting for imaging two wavelengths simultaneously (uses side illumination)
Fig.3 (right) Setting for imaging one wavelength by inserting a light guide (uses epi-illumination)





Dual DLFLSP2R (can use both Epi and Side Illumination)




Combination with THT component (can use both Epi and Side Illumination)




DLFLSP2R without THT component (uses only side illumination)




Combination with THT-sideways (can use both Epi and Side Illumination)




DLFLSP2R without THT component (uses only side illumination)




Combination with Inverted THT



Specifications

Filter Size Dichroic Mirror (x1): 50mmx70mm
Emission Filter: 50mm diameter
Lenses Leica Microsystems PLAN APO lenses
(Attached to top, bottom and left side.)
Positioning mechanism Two Kinematic Mount Adjustment Knobs
Applicable Microscope Brainvision's tandem-lens macroscope (THT series)

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