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High-Powered LED Illumination System LEX2-LZ4

Overview

LEX2-LZ4 is a new LED illumination system using a high-powered LED chip. It has been designed to offer brighter and more stable lighting than the previous LED system. There are two models available: blue (peak: 465nm) and green (peak: 530nm).

Description

LEX2-LZ4 features a high-powered LED chip and original feedback mechanism for maintaining stable light intensity. It is our best LED system in terms of brightness and stability, resulting in very good signal to noise ratios.

LEX2-LZ4 can be used for many biological applications. It can be used with MiCAM high speed imaging systems as an excitation light source for detecting fluorescent signals, or as a light stimulator for cells that express light-activated proteins in electrophysiological experiments.

Features

High Power

LEX2-LZ4 consists of a high-powered LED chip, which produces a light intensity over 360mW/cm2. It is approximately 2.3 times brighter than 150W halogen light source.

The intensity can be simply adjusted with coarse/fine dials on the front panel in the range between 0 and 150% (100% to 150% intensity can be used for short-pulse illumination lasting less than 1 second).


Comparison of light intensity (mW/cm2) between LEX2-LZ4-G green LED Light system and 150W halogen lamp system (with green filter) at 100% intensity.



High Stability of Light Intensity through Feed-Back Mechanism

The intensity is more stable than halogen lamps and does not produce any flicker or large arc fluctuations, in comparison to Xenon and Mercury light sources. LEDs are an ideal illumination source for high speed imaging. However, light intensity of LEDs tends to decrease when turned on for long periods of time, due to the heat of LED chips. To keep light intensity constant, LEX2-LZ4 adopts an original feedback technology. This feedback mechanism results in less than ±0.3% drift within 30 seconds at 50 % intensity.


Change in light intensity within 30 seconds at 50% intensity. Measured with fast CCD imaging system, MiCAM02-HR (30 seconds at 100 frames/second, 4 times averaging).




Synchronized Illumination with External Signal

The light can be turned ON/OFF by signals from external devices such as MiCAM high speed imaging systems or pulse generators. By sending a pulse greater than 1.5V through a BNC connector to the front panel of MiCAM high speed imaging systems, the light can be activated. LEX2-LZ4 does not use any mechanical shutter, so there is no noise caused by vibration or sound.

Adjustment of the light intensity by External signal

Light intensity can be controlled by analog voltage signal from external device. 3.3V voltage input can set about 100% light intensity.

Monitor function for light intensity setting

LED intensity adjustment control can be monitored through a BNC connector. At 100% intensity setting, outputted voltage is 3.3V.


Applications

  • Excitation light source for MiCAM high speed fluorescence imaging systems using indicators including voltage-sensitive dyes, calcium indicators, and genetically encoded optical probes such as voltage-sensitive proteins.
  • Light source for stimulation to light-activated protein such as channelrhodopsin 2 (ChR2) and halorhodopsion (NpHR).
  • Other imaging applications requiring stable and bright light.

LED Wavelength


Dimension



Specifications

Item LEX2-LZ4-B LEX2-LZ4-G
Center Wavelength 465 nm 530 nm
LED intensity
at 100% setting (*1)
approx. 410 mW/cm2 approx. 360 mW/cm2
LED intensity
at 150% setting (*1)
approx. 490 mW/cm2 approx. 410 mW/cm2
Light Fluctuation (*2) approx. <±0.015%
Change in light intensity
in 30 seconds (*2)
approx. <±0.3%
Intensity stabilization system PD feedback circuit
Shutter Control Manual control: Manual switch on the front panel
External control:triggered by over 1.5V input
Intensity Control Manual control: 0-100%, 100%-150% (only for short-pulse illumination)
External control: 0-100% control by voltage input (100% intensity by 3.3V input)
Monitoring Output Output of voltage signal (3.3V output at 100% intensity)
Protection Function Overcurrent protection, overheat cutoff
Light Guide Option
Environmental Conditions Indoor use, temperature 0℃ to 35℃ Humidity 0 to 70%
Cooling Methods By fan
Dimensions and Weight 150mm(W) x 250mm(D) x 83mm(H) / 2kg
Input Voltage and Power Consumption 100-220V, 30W
(*1) Measured value at output end of straight light guide (glass type, bundle diameter of 10mm, length of 2200mm ).
(*2) <50% intensity. Measured with fast CCD imaging system, MiCAM02-HR (30 seconds at 100 frames/second, 4 times averaging).

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