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FAQ

What is MiCAM?
MiCAM is the high speed camera series that is specifically designed to detect very small changes in light levels at optimal high speeds, high resolution, and high signal to noise ratios. All of the cameras are designed and manufactured in Japan.
How many models are there in the MiCAM series?
The CMOS based MiCAM ULTIMA and CCD based MiCAM02 are currently available. Two types of cameras (or sensors) are available for MiCAM ULTIMA. The difference between the two cameras is the well depth, or light acceptance, of the sensor. ULTIMA-H well depth is 10 million electrons, and ULTIMA-L well depth is 1.5 million electrons. Generally, we recommend ULTIMA-L for low to medium light applications and ULTIMA-H for high light level applications. Two types of CCD cameras are available for MiCAM02, HR camera with 1/2" CCD and HS camera with 1/5"CCD. Both cameras have certain advantages over each other with regards to imaging area size, and temporal and spatial resolution.
What is membrane voltage-sensitive dye?
Membrane voltage-sensitive dye changes its fluorescence intensity according to membrane potential.
What is optical mapping of membrane potential?
Optical mapping is based on traditional electrophysiology, but the optical imaging method is used to further understand membrane potential in cell networks over both space and time. Membrane potential is recorded optically in two dimensions using voltage-sensitive dye, which also differs from patch recordings of membrane potential activity using conventional electrodes. The optical mapping method allows researchers to image a large area with minimal invasiveness.
What kinds of biological samples can be used for optical mapping?
The optical mapping techniques have been used for in vivo animals, in vitro samples such as tissue slices, isolated muscle tissues and cultured cells, and similar applications.
What is the maximum frame rate available?
MiCAM ULTIMA can image up to 10,000frames/second (10 kHz), and MiCAM02 can image at 1,000frames/second (1 kHz, HS camera).
What is needed for the optical recording of membrane potential?
The MiCAM system includes a camera, processor, data acquisition software, data analysis software, software license, personal computer with interface, and PC monitor. Additional equipment, such as a fluorescence microscope, bright and stable-light source, anti-vibration table, and isolator, etc., are also necessary depending on the type of experiments.

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