Sample Data

Calcium Imaging of Cultured Cardiomyocyte Sheet

Sample: Cultured cardiomyocyte sheet
Fluorescence Dye: Calcium Dye (Cal-520)
Imaging System: MiCAM05-C35IR
Pixels: 636x360
Frame Rate: 200fps (5msec/frame)
Provided by: Dr. Hiroko Izumi-Nakaseko, Department of Pharmacology, Faculty of Medicine, Toho University

High-Speed Calcium Imaging of Isolated Mouse Atrial Cardiomyocytes

Sample: Freshly isolated mouse atrial cardiomyocytes. Cells were plated on glass cover slips coated with laminin and used on the same day as isolation.
Method: Cells were paced by electrical field applied through a perfusion solution. Imaging was performed on Nikon DIAPHOT 300 microscope with 20X lens and SPECTRA-X Lumencor light source.
Fluorescence Dye: Calcium sensitive dye (Fluo-4 AM)
Imaging System: MiCAM05-ULTIMA
Pixels: 100x100
Frame Rate: 200fps (5.0msec/frame)
Provided by: Dr. Roman Medvedev and Dr. Alexey Glukhov, Department of Medicine, University of Wisconsin-Madison

Monolayer of neonatal mouse ventricular myocyte stained with voltage sensitive dye

Sample: Monolayer of neonatal mouse ventricular myocyte. Sample was scratched with a needle tip at three locations (visible on the fluorescence pictures) to induce conduction blocks and meandering of the wavefront.
Method: Sample is paced locally at different frequencies using a glass microelectrode positioned at the edge of the sample
Fluorescence Dye: Voltage sensitive dye (Di-8-Anepps)
Imaging System: MiCAM03-N256
Pixels: 256x256
Frame Rate: 1,000fps (1.0msec/frame)
Provided by: Dr. Jan Kucera and Dr. Ange Maguy, Department of Physiology, University of Bern

Calcium Imaging of Cultured Cardiomyocytes (Fluo-4)

The preparation was cultured rat cardiomyocytes stained with Fluo-4 and activity was recorded using MiCAM01 (old model) in 2002. The current model, MiCAM02 can significantly improve the data in terms of spatial resolution, sensitivity and S/N ratio.

Voltage-Sensitive Dye Imaging of Cultured Neonatal Rat Ventricular Cardiomyocytes

Sustained spiral wave reentry in a 3 day old monolayer disc of cultured neonatal rat ventricular cardiomyocytes (frequency ~ 5 Hz; diameter of the preparation = 8 mm). The preparation was stained with the voltage sensitive dye di-8-ANEPPS and activity was recorded at 1,000 frames/second using a custom-made tandem lens macroscope and an Ultima-L camera system.

Provided by: Dr.Stephan Rohr, University of Bern

Voltage-sensitive dye changes its absorbance and fluorescence intensity when membrane potential changes in a stained brain or heart tissue.

By using voltage-sensitive dyes as chemical probes and capturing changes in light intensity with the use of a high-speed imaging device, it is possible to image in real time the activity of where, when, and how much excitation or inhibition occurred, in the brain and heart.

Product suitable for this kind of application

MiCAM03-N256 is a high-speed imaging system that captures and visualizes small changes in fluorescence intensity from biological samples stained with fluorescent probes, such as voltage sensitive dyes and calcium dyes.

Spatial Resolution :: 128x128 - 256x256 pixles
(32x32 pixels - 256x256 pixles with option)
Maximum Frame Rate :: 1,000fps
(20,000 fps available with option)
Up to 2 camera heads can be used with completely synchronization.

MiCAM03-C35IR is an imaging system equipped with a large 35mm full-size CMOS sensor, which has a spatial resolution of 2,160x1,280 pixels.

This model uses an ultra-high sensitive CMOS sensor which has large pixels, uses low noise technology, and has > 80% quantum efficiency. This makes it possible to image dim fluorescent samples at wide fields of view.

Wide Field of View :: 35mm full-size CMOS sensor
(47.7 mm diagonal)
Spatial Resolution :: 2,160x1,280 pixels
Maximum Frame Rate :: 277.8 fps at 1,212x360 pixels

Activity of cardiomyocytes in a culture plate can be measured without staining and invasion.

Result is displayed in real time in a waveform, and number of beats and contraction time are automatically detected with a single button.

Automatic detection of cardiomyocyte beating and contraction time
Just place a culture plate without fluorescent dye and electrode
Real-time automatic detection for multiwell plate