Sample Data
High Speed Voltage Sensitive Dye Imaging of Spontaneous Activities in Cultured Neuronal Network
- Sample
- Cultured Neurons
- Fluorescence Dye
- FluoVolt
- Imaging System
- MiCAM05-C35IR
- Pixels
- 636x360
- Frame Rate
- 277fps (3.6msec/frame)
Voltage Sensitive Dye Imaging of Brain Slice (MiCAM05-N256)
- Sample
- Brain Slice
- Method
- Electrical stimulation
- Fluorescence Dye
- Voltage sensitive dye (Di-4-Anepps)
- Imaging System
- MiCAM05-N256
- Pixels
- 256x256
- Frame Rate
- 1,000fps (1.0msec/frame)
- Provided by
- Dr. Takashi Tominaga, Institute of Neuroscience, Tokushima Bunri University
Voltage-Sensitive Dye Imaging of Mouse Brain Slice (MiCAM ULTIMA)
- Sample
- Mouse Brain Slice including Cortex and Hippocampus
- Method
- Electrical stimulation
- Fluorescence Dye
- Voltage sensitive dye (Di-4-Anepps)
- Imaging System
- MiCAM05-D225
- Pixels
- 256x240 pixels
- Frame Rate
- 1,000fps (1.0msec/frame)
- Provided by
- Dr. Takashi Tominaga, Institute of Neuroscience, Tokushima Bunri University
Mouse In Vivo Voltage-Sensitive Dye Imaging (MiCAM ULTIMA-H)
This data was obtained from a 2 month-old mouse that was under urethane anaesthesia (1.75 g/kg). A heating pad was used to maintain the measured body temperature of the mouse at 37°C. Two electrodes were inserted under the skin of the forearms to receive the electrocardiogram signal which was used to trigger the MiCAM ULTIMA acquisition.
- Sample
- Mouse In Vivo Bain (Somatosensory Barrel Cortex)
- Method
- Piezo Whisker Flick Stimulation
- Fluorescence Dye
- Voltage sensitive dye (RH-1691)
- Imaging System
- MiCAM ULTIMA
- Pixels
- 100x100 pixels
- Frame Rate
- 500fps (2.0msec/frame)
- Provided by
- Dr.Isabelle Ferezou and Dr.Carl C.H. Petersen, Brain Mind Institute, EPFL, Switzerland
- Reference
- Visualizing the Cortical Representation of Whisker Touch: Voltage-Sensitive Dye Imaging in Freely Moving Mice.
Neuron. 2006 May 18;50(4):617-29.
Rat In Vivo Optical Mapping using Voltage-Sensitive Dye (MiCAM02-HR)
- Sample
- Rat In Vivo Bain (Insular Cortex)
- Method
- Electrical stimulation (a train of 5 stimuli with 20msec interval)
- Fluorescence Dye
- Voltage sensitive dye (RH-1691)
- Imaging System
- MiCAM02-HR
- Frame Rate
- 250fps (4.0msec/frame)
- Provided by
- Dr.Satoshi Fujita and Dr.Masayuki Kobayashi, Nihon University School of Dentistry, Japan
- Reference
- Spatiotemporal dynamics of excitation in rat insular cortex: intrinsic corticocortical circuit regulates caudal-rostro excitatory propagation from the insular to frontal cortex.
Neuroscience. 2009 Oct 1.
Rat Cortex Slice Optical Mapping using Voltage-Sensitive Dye (MiCAM02-HR)
Membrane potential imaging of rat cortex slice with MiCAM02-HR camera. Temporal resolution is 384x256 pixels and frame rate is 7.0msec/frame.
- Sample
- Rat Cortex Slice
- Method
- Electrical stimulation
- Fluorescence Dye
- Voltage sensitive dye Di-4-ANEPPS
- Imaging System
- MiCAM02-HR
- Frame Rate
- 143fps (7.0msec/frame)
- Provided by
- Dr.Takashi Tominaga and Dr.Michinori Ichikawa, Brain Science Institute, RIKEN
Ultra High Speed Optical Mapping (10,000fps) of Rat Hippocampus Brain Slice
Action potential propagation was recorded at 0.1msec/frame. 10,000fps recording allows us to identify presynaptic activity and postsynaptic activity separately.
- Sample
- Rat Hippocampus Slice
- Method
- Electrical stimulation
- Fluorescence Dye
- Voltage sensitive dye Di-4-ANEPPS
- Imaging System
- MiCAM ULTIMA
- Frame Rate
- 10,000fps (0.1msec/frame)
- Provided by
- Dr.Takashi Tominaga and Dr.Michinori Ichikawa, Brain Science Institute, RIKEN
Product suitable for this kind of application
- High Speed Imaging System
- MiCAM03-N256
MiCAM03-N256 is a high-speed imaging system that captures and visualizes small changes in fluorescence intensity from biological samples stained with fluorescent probes, such as voltage sensitive dyes and calcium dyes.
- Spatial Resolution :
- 128x128 - 256x256 pixles
(32x32 pixels - 256x256 pixles with option) - Maximum Frame Rate :
- 1,000fps
(20,000 fps available with option) - Up to 2 camera heads can be used with completely synchronization.
- Ultra-High Sensitivity, Wide-Field Imaging System
- MiCAM03-C35IR
MiCAM03-C35IR is an imaging system equipped with a large 35mm full-size CMOS sensor, which has a spatial resolution of 2,160x1,280 pixels.
This model uses an ultra-high sensitive CMOS sensor which has large pixels, uses low noise technology, and has > 80% quantum efficiency. This makes it possible to image dim fluorescent samples at wide fields of view.
- Wide Field of View :
- 35mm full-size CMOS sensor
(47.7 mm diagonal) - Spatial Resolution :
- 2,160x1,280 pixels
- Maximum Frame Rate :
- 277.8 fps at 1,212x360 pixels