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  3. BV-XB1: Overview

Neuronal Imaging BV-XB1

BV-XB1 is a high resolution imaging system which combines a high resolution camera with 1920 x 1200 pixels, plus image acquisition software developed for bioimaging experiments.

The system includes ESTM-9, which is equipped with external camera control, external illumination control, pulse outputs, analog outputs, and analog inputs recording functionality. By connecting external equipment such as a light source, electrical stimulator, and amplifiers, ESTM-9 synchronizes image acquisition with inputs/outputs of external equipment.

In combination with Brainvision’s fluorescence macroscope, it is possible to construct a wide-field / high resolution / high S/N ratio fluorescence imaging system.

XB1CM Camera Head

XB1 Camera Head

The dark noise of the CMOS sensor used in the XB1CM camera head is as low as that of CCD sensors, and it is possible to capture images at relatively high frame rates. Due to this combination of low noise and relatively high speed, XB1CM is suitable for low light level bioimaging applications, such as calcium imaging, imaging using fluorescent proteins such as GCaMP/FRET, and flavoprotein intrinsic imaging.

High Resolution, Large Sensor and Wide Field of View

Spatial resolution: 1920x1200 pixels, sensor size:11.3mmx7.1mm

Total Magnification
of Macroscope
Field of View
0.63x 17.9mm x 11.2mm
1.0x 11.3mm x 7.1mm
1.6x 7.1mm x 4.4mm

Wide Dynamic Range and Low Dark Noise

A large well capacity of about 32ke- results in greater dynamic range than CCD sensors, and low dark noise comparable to a CCD camera. It can be used for wide range of applications from low to medium light conditions.


Maximum frame rate and sensitivity can be changed by number of pixels and binning

Binning Resolution Maximum frame rate
(fps)
  Frame interval    
(ms)
1x1 1920x1200 Full pixel readout 78.11 12.8
960x600 Partial readout 249.44 4.0
640x400 362.84 2.8
480x300 469.48 2.1
384x240 570.13 1.7
2x2 960x600 Full pixel readout 128.77 7.8
480x300 Partial readout 249.44 4.0
240x150 469.48 2.1
3x3 640x400 Full pixel readout 128.77 7.8
320x200 Partial readout 249.44 4.0
160x100 469.48 2.1
4x4 480x300 Full pixel readout 128.77 7.8
240x150 Partial readout 249.44 4.0
192x100 307.03 3.3
160x100 362.84 2.8

(*1) Size of effective pixel will change, dependent on binning.
(*2) Size of effective sensor area and size of effective pixel will change, depending on selected number of pixels and binning.





ESTM-9

ESTM-9

By connecting external equipment such as camera, light source, electrical stimulator, and amplifiers, ESTM-9 can synchronize image acquisition with inputs/outputs of external equipment.


Acquisition Control of External Camera

  • Outputs frame timing pulses to external camera, or receives frame timing signals from external camera.
  • Time-lapse acquisition control is also available.
Acquisition Control


Light Source Control

  • Outputs signals to external light source in synchronization with camera control.
  • Continuous, intermittent, or custom light pattern available.

Pulse Stimulator

  • 4 channels of pulse stimulation can be controlled individually.
  • Stimulation works with or without camera control.
  • Continuous stimulation using free-run mode is also available.
Pulse Stimulator


Analog Signal Recording

  • 2 channels of analog inputs.
  • Pre-trigger, delayed-trigger, and multiple trigger source available.



Other Features

Turnkey System Ready for Immediate Use

The system consists of hardware/software specifically designed for high-speed fluorescence imaging, and the PC and monitor are included as standard equipment. Before use, calibration/setting/tuning is not necessary. The hardware connection is straightforward between components using cables, making it possible to acquire data immediately after setup.

Diagram

Multi Camera System

By connecting multiple XB1CMs to PC, it is possible to operate as a fully synchronized multi camera system. Two images can be superimposed on monitor display so that user can overlap two images.


Time Lapse Imaging

BV-XB1 can capture phenomenon that changes in a few minutes up to several hours, intermittently. Because illumination light is turned on only at the time of image acquisition, phototoxic effects to sample are minimized, and a stable baseline can be acquired.
Time LapseExample of time-lapse imaging




Software

Acquisition/Analysis Software (BV Workbench)

BV Workbench is the image acquisition software that controls BV-XB1 imaging system. In addition to image acquisition and control of inputs and outputs of the imaging system, analysis functions including movie playback, wave display, filter functions, etc…are also included. BV Workbench has an intuitive user interface that is easily operated with the use of a mouse.

BV Workbench

System
Name High Resolution Imaging System BV-XB1
Model BV-XB1
Standard System
Configuration
XB1 camera head (XB1CM), ESTM-9,
Cables, PC, Monitor
Acquisition software, Analysis Software
Supported OS Windows 7/10 64bit
Camera Head
Name XB1 Camera Head
Model XB1CM
Image Sensor SONY IMX174 CMOS (1/1.2inch)
Resolution (H x V) 1920 x 1200
Active Image Area (H x V) 11.3mm x 7.1mm
Pixel Size (H x V) 5.86um x 5.86um
Shutter Mode Global Shutter
Maximum Frame Rate
and Pixels
78.11fps@1920x1200pixels
249.44fps@960x600pixels
362.82fps@640x400pixels
469.48fps@480x300pixels
570.13fps@384x240pixels
Quantum Efficiency 70%
Dark Noise 6.6e-
Saturation Capacity 31.7ke-
Dynamic Range 73.7dB
Pixel Bit Depth 12bit
Interface USB3.0
Lens Mount C-mount
Dimension 29.3mm x 29mm x 29mm
Weight 80g
ESTM-9
Pulse Output 4 ch (BNC)
Analog Input 2 ch (BNC)
Analog Output 2 ch (BNC)
Camera I/O Port for XB1CM 2 ch (4 pin connector)
Frame Timing Signal Output 1 ch (BNC)
Acquisition Status Output 1 ch (BNC)
Shutter Control Output 1 ch (BNC)
External Trigger Input 1 ch (BNC)
Others Trigger button, Stop button,
8-pin connector for optional function
Control Software 2 versions
Interface USB2.0
Power USB bus power
Includes ESTM-9, USB2.0 cable, Software
Dimensions 180mm x 120mm x 65mm
Each function
Main Trigger Trigger Mode Software / External / Internal
Trigger Source Software / External Trigger Input / Trigger Button
Trigger Edge Positive / Negative
Pulse Output Output Voltage 5V
Clock Cycle 10μsec - 10msec
Duration time 10μsec - 10min
Delay Time 10μsec - 10min
Interval Time 10μsec - 10min
Pulse Number 1 - 65,535
Analog Input Input Range 1V / 3V / 10V
Sample Clock 10μsec - 10msec
Trigger Source Main / Pulse1 / Pulse2 / Pulse3 / Pulse4 / Ain1 level
Others Previous-trigger, Delayed-trigger,
Low-pass filter, Polarity inversion
Analog
Output
Output Voltage -10V - 10V
Output Waveform Sine, Triangle, Sawtooth,
Custom (with a CSV file)
Camera Control Number of Camera Port Brainvision BV-XB1 : 2 (I/O connectors)
Other camera : 1 (BNC connectors)
Frame Timing Signal Output or Input
Frame Timing Signal Cycle 10μsec-300msec
Trigger Source Main/Ain1 level/Pulse3
Others Time lapse
Light Control Lighting Mode Continuous
Synchronous with data acquisition /
Synchronous with frame acquisition /
Custom lighting pattern
Others Polarity inversion
  • Wide-field calcium imaging
  • Intrinsic optical signal (IOS) imaging
  • GCaMP imaging
  • FRET imaging
Neuronal Imaging