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THT Macroscope

The THT is a fluorescence widefield macroscope developed for detecting low light fluorescence that is emitted from biological sample at a low magnicifation. This macroscope has a very simple optical path combining two large-diameter objective lenses and a custom-made large fluorescent filter.

Custom-made systems can be constructed according to your request.


Low Magnifications with High N.A Values:
Detecting Low Light Fluorescence with a Wide Field of View

Depending on the combination of lenses, the total magnification is about 0.19x to 6.3x (Actual field of view varies depending on the sensor size of the camera used).

Because a large diameter and high numerical aperture lens is used, extremely bright imaging is possible. Signals that were previously difficult to detect may be easier to detect, and even samples that could be captured so far may have higher S/N ratios.

Objective lens with large diameter and long working distance
Objective
Magnifications
Lens Diameter Working Distance
0.3x 50mm 140mm
0.63x 43mm 67mm
1x 60mm 62mm
1.6x 59mm 31mm
2x 57mm 20mm
5x 39mm 19mm
Projection lens with aperture/focal length adjustment function
Projection
Lens
Magnification Mount
50mm 0.63x C
85mm/1.4 1.0x EF/T/C
135mm/2.0 1.6x EF/T/C



Simple Structure: Specializing in Imaging

There is no visual observation function. It is designed to focus on camera shooting with a higher S/N ratio.


The optical axis can be tilted and rotated: no need to tilt the sample

A stand that can rotate the optical axis 360 degrees left and right is also selectable. By tilting the optical axis, it is possible to image the entire temporal lobe of the brain without tilting the animal sample.

  



Easy Removal of Fluorescent Filter

The fluorescent filter is held in a removable filter cube. the fluorescent filter can be changed by replacing the filter cube.



Multifunction: Used As a Fluorescence Beam Splitter

Normally, an excitation light source is connected to the left port of the THT main unit and used as a coaxial epi-illuminator.

In addition, it can be used as a two-wavelength fluorescence splitting device. In this case, the same lens and camera as the upper port are connected to the left port, and two-wavelength fluorescence simultaneous measurement using two cameras is performed.



Inverted type is also possible

An inverted fluorescence macroscope has been manufactured. The space on the sample stage becomes freer, so we expect that the degree of freedom in manipulator installation and electrode operation will increase. It can also be used with an upright fluorescent microscope. Please feel free to ask us for microscope bases and sample stages to suit the purpose of the experiment.


Applications

  • Membrane potential imaging using voltage-sensitive dyes
  • Widefield calcium imaging
  • Intrinsic optical signal (IOS) imaging
  • Imaging FRET, GCaMP, and various fluorescent proteins such as GEVI
  • Ratiometric fluorescence imaging with multiple camera heads

Epi illumination/single wavelength imaging

Side illumination/dual wavelength imaging


Epi and side illumination
dual wavelength imaging, Upright

Epi and side illumination
dual-wavelength imaging, 90 degrees rotation

Epi-illumination
Single wavelength imaging
Brainvision XY stage included

Boom-arm stand

Extension arm

Olympus BX51WI base
includes manual XY table from Luigs&Neumann, Germany

Interted/dual wavelength imaging

Horizontal imaging for Langendorff perfused heart

High rigid arm type for In Vivo imaging

Specifications

Model Fluorescence Macroscope THT Series
Optical System Tandem lens optical system used for camera only
Focusing device Manual coarse/fine focus drive or
Motorized coarse/fine focus drive (incl. dial type controller)
Microscope carrier Objective nosepiece (two M65 ports), or tilting mount
Filter Cube Detachable
One 50mm diameter filter can be installed on the top and side
Dichroic mirror mount with 2-axis tilt adjustment mechanism
Illumination Light for Excitation Coaxial epi-illumination or side illumination
Lens port Top: 1, Side: 1, Bottom: 1
Objective lens PLAN 0.3x
PLAN APO 0.63x
PLAN APO 1x
PLAN APO 1.6x
PLAN APO 2x
PLAN APO 5x
Projection lens PLAN APO 0.63x
PLAN APO 1x
PLAN APO 1.6x
PLAN APO 2x
135mm/2.0 lens (w/ aperture and focus adjustment function)
85mm/1.4 lens (w/ aperture and focus adjustment function)
50mm/0.95 lens(w/ aperture and focus adjustment function)
Sample stage Option

Approximate magnification and field size for N256 camera and THT series

Objective
Lens
Condensing Lens (Camera Side)
0.63x 1x 1.6x 50mm
(∞ setting)
85mm
(∞ setting)
135mm
(∞ setting)
0.3x
(WD:141mm)
0.48x
(36.6mmx36.6mm)
0.3x
(58.6mmx58.6mm)
0.19x
(92.6mmx92.6mm)
- - -
0.63x
(WD:67mm)
1x
(17.6mmx17.6mm)
0.63x
(27.9mmx27.9mm)
0.4x
(44mmx44mm)
0.4x
(44mmx44mm)
0.69x
(25.5mmx25.5mm)
1.27x
(13.9mmx13.9mm)
1x
(WD:61.5mm)
1.6x
(11mmx11mm)
1x
(17.6mmx17.6mm)
0.63x
(27.9mmx27.9mm)
0.64x
(27.5mmx27.5mm)
- 2x
(8.8mmx8.8mm)
1.6x
(WD:30.5mm)
2.6x
(6.7mmx6.7mm)
1.6x
(11mmx11mm)
1x
(17.6mmx17.6mm)
1.02x
(17.6mmx17.6mm)
- -
2x
(WD:20.1mm)
3.2x
(5.5mmx5.5mm)
2x
(8.8mmx8.8mm)
1.26x
(13.9mmx13.9mm)
1.28x
(13.7mmx13.7mm)
2.23x
(7.9mmx7.9mm)
4x
(4.4mmx4.4mm)
5x
(WD:19mm)
6.3x
(2.8mmx2.8mm)
4x
(4.4mmx4.4mm)
2.5x
(7mmx7mm)
- 4.48x
(3.9mmx3.9mm)
-
* Blank is no measured data
Elina A.K. Jacobs, Nicholas A. Steinmetz, Andrew J. Peters, Matteo Carandini, Kenneth D. Harris  

Cortical State Fluctuations during Sensory Decision Making.

Curr Biol . 2020 Dec 21;30(24):4944-4955.e7.


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