Multi-LED Light System LEX9

Higher power : Improve S/N ratios

The new LED chip has a higher output power of more than 4.3 times that of a commercially available 150W halogen light source and 1.8 times that of our old LED system.

LEX9 is essential for improving S/N ratios in wide-field imaging experiments where excitation light density tends to decrease and in high-speed imaging experiments where exposure times are short.

• Comparison of brightness of three different light sources.
  Wavelength is around 530nm and light intensity setting is 100%.
  
  Left: 150W halogen light source (Uses 531nm bandpass filter)
  Center: LEX3-G green LED light system
  Right: LEX9 LED light system (530nm LED unit installed)

Highly stable : Minimize unnecessary noise when imaging biological signals

Generally, when an LED is turned on for a long time, light intensity decreases as the temperature rises. In the case of biological imaging, it is necessary to capture small changes in light intensity. However, drifting of excitation light can become a major source of noise. Although drift can be removed in some cases through software processing, it can also impair the accuracy of biological signals.

LEX9 is equipped with an optical feedback mechanism that monitors the intensity of the output light and keeps the intensity constant, minimizing drift.

• Light intensity change in 5 seconds is less than 0.3% ("OFB"=optical feedback)

• Light intensity change after 10 minutes is less than 0.5% ("OFB"=optical feedback)

• Light intensity change after 100 minutes is less than 1% (“OFB"=optical feedback”)

Dual wavelength excitation : Two wavelengths can be acquired with one camera

Two LED units can be installed inside LEX9.
Light from the two LED units is output from the same optical path/light guide via an internally installed dichroic mirror. There is no need to prepare a separate beam splitter to connect to the microscope.

It is ideal as an excitation light source for GCaMP imaging using 405nm and 460nm, as well as other dual-wavelength imaging experiments.

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Two-wavelength excitation for GCaMP imaging using LEX9 and MiCAM

Fast switching : For high-speed imaging and pulsed light stimulation

Since no mechanical mechanism is used, such as a filter wheel, it is possible to switch between two wavelengths at high speed. Even in applications where each frame is illuminated with excitation light of a different wavelength, it is possible to increase the camera frame rate more than ever before.
(Switching between two wavelengths requires an external pulse generator or camera system that can output two types of pulse patterns.)

Expandability : Compatible with various fluorescent probes

It is possible to purchase additional LED units with different wavelengths. Therefore, customers can easily add or replace LED units by themselves. We are also planning to develop a white light LED unit.

• LED unit

• Center wavelengths of available LED units
  405nm / 460nm / 530nm / 580nm / 620nm (Option: 500nm / 730nm / 780nm / 875nm / 940nm)

• It is possible to change the wavelength by replacing the internal LED unit.

Fluorescence filters are held in a filter cube (shown in the image below).
Standard size φ25mm bandpass filters and 25mmx36mm dichroic mirror can be installed in the filter cube, allowing customers to freely and easily replace the fluorescence filters.

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Wide area illumination : Illuminate entire area at low magnifications

A φ10mm light guide is used as our standard light guide to brightly illuminate a wide area. Suitable for fluorescence imaging at low magnifications and wide fields of view.

Extremely short rise time (< 10μs) suitable for pulsed light irradiation

e.g. for optogenetics experiments using channelrhodopsin.

Automatic calibration function that sets the optimal light output conditions for each LED unit

No need to change internal settings even after changing the LED unit

Microscope adapter available (sold separately)

Can be connected to microscopes from various companies

Can also be operated with remote control (sold separately)

We also plan to develop an option that allows users to operate the system from a web browser on a smartphone or PC.

Wavelength characteristics of LED units

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Wavelength characteristics of LED units and transmission wavelength range of bandpass filter

• The wavelength characteristics of each LED are shown with each intensity peak as 100%. Actual LEDs have a slight wavelength shift due to individual differences. Therefore, it is not possible to compare the intensity of each wavelength of different LEDs in the above figure. The transmission range of a bandpass filter is expressed by the width of each color block. We propose two types of bandpass filters: narrowband (N) and wideband (W) for deep blue (DB), blue (B), green (G), yellow (Y), and red (R) LEDs. We recommend bandpass filters with a thickness of 3.5mm.

Recommended optical filters

If only one wavelength is used, a broad bandpass filter can be selected. If two wavelengths are used alternately or simultaneously, a narrow bandpass filter can be selected so the transmitted wavelengths do not overlap.

Filters not listed below can be used as long as they are standard sizes (φ25mm bandpass filter and dichroic mirror of approximately 25mmx36mm).

	LED unit	Name	Code	Application
	(A) 405nm	Narrowband BP405nm	DBN405/20c	Autofluorescence, GCaMP (for control), others
	①	Dichroic mirror DM435	DM435e	(A) // (B)(C)(D)(E)(F)
	(B) 460nm	Narrowband BP466nm	BN466/40e	GCaMP, ChR2
		Wideband BP460nm	BW460/60s	FITC, Fluo-4, FluoVolt, others
	②	Dichroic mirror DM495	DM495e	(A)(B) // (C)(D)(E)(F)
	(C) 500nm	Narrowband BP500nm	BN500/24e	ANNINE-6
		Wideband BP503nm	BW503/40s
	(D) 530nm	Narrowband BP525nm	GN525/45e	RH237, Rhod-2, others
		Wideband BP520nm	GW520/70e	Di-4-ANEPPS, Di-2-ANEPEQ, others
	③	Dichroic mirror DM552	DM552w	(A)(B)(C)(D) // (E)(F)
	(E) 580nm	Narrowband BP586nm	YN586/20w	NpHR, others
		Wideband BP585nm	YW586/40e
		Wideband BP582nm	YW582/64s
	④	Dichroic mirror DM605	DM605e	(A)(B)(C)(D)(E) // (F)
	(F) 620nm	Narrowband BP628nm	RN628/32e	RH-1691, Di-4-ANBDQBS, others
		Wideband BP620nm	RW620/60e

Item	Description	Memo
Model	LEX9
Name	High-powered Multi LED Light System LEX9
LED unit
Center wavelength	405nm / 460nm / 530nm / 580nm / 620nm (Option: 500nm / 730nm / 780nm / 875nm / 940nm)	- Width of output wavelength depends on the bandpass filter used together. - Please contact us for wavelengths not listed.
LED intensity at 100% setting	405nm:   407mW/cm2 460nm: 1,833mW/cm2 530nm:   650mW/cm2 630nm: 1,057mW/cm2	Depends on the transmission rate of the bandpass filter used together.
Number of LED units that can be used simultaneously	2
Number of built-in filter cube	1	2 excitation filters / 1 dichroic mirror
Compatible excitation filter	25m diameter, 3.5mm thickness
Compatible dichroic mirror	25mmx36mm
Main body
Intensity control	Manual control, or Remote controller (sold separately)	We also plan to develop an option for users to operate the system from a smartphone or PC web browser.
Lighting control	Manual control: key switch on the front panel External control: triggered by over 1.5V input
Lighting mode	CC (constant current) mode OFB (optical feedback) mode	Ideal for pulsed light irradiation Ideal for normal use
Drift of light intensity	< 0.3% (5 sec) < 0.5% (10 min) < 1.0% (100 min)	with OFB mode
Rise time	< 10μs	with CC mode
Frequency characteristic	<= 100kHz (10μs) @ CC mode <=  20kHz (50μs) @ OFB mode
Intensity stabilization system	PD feedback circuit
LED protection system	Overcurrent suppression circuit and overheat suppression circuit
Cooling method	Heat sink and electric fan
Safety mechanism	Operation control by key Interlock system (light guide connector ) Manual reset after optical output is cut off Emergency stop switch (optional)
Recommended light guide (sold separately)	For epi-illumination: Moritex MSG10-2200S For side illumination: Moritex MSG10-2200S	Please contact us for other light guides.
Microscope adapter (sold separately)	Brainvisin's Fluorescence Mesoscope Fluorescence microscopes manufactured by Olympus/Nikon/Leica/Zeiss
Environmental Conditions	Indoor use, temperature 0℃ to 35℃ Humidity 0 to 70%
Dimensions / Weight	300mm(W) x 350mm(D) x 130mm(H) / 7.3-7.6kg
Input Voltage / Power Consumption	100V-220V 200W

* Specifications and appearance are subject to change without prior notice due to continuous improvements.
* This product is made in Japan. * This product is for research purposes only.