THT Mesoscope for Fluorescence Imaging

Low Magnifications with High N.A Values:
Detecting Low Light Fluorescence with a Wide Field of View

Depending on the combination of lenses, the total magnification is about 0.19x to 6.3x (Actual field of view varies depending on the sensor size of the camera used).

Because a large diameter and high numerical aperture lens is used, extremely bright imaging is possible. Signals that were previously difficult to detect may be easier to detect, and even samples that could be captured so far may have higher S/N ratios.

Simple Structure: Specializing in Imaging

There is no visual observation function. It is designed to focus on camera shooting with a higher S/N ratio.

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The optical axis can be tilted and rotated: no need to tilt the animal sample

A stand that can rotate the optical axis 360 degrees left and right is also selectable. By tilting the optical axis, it is possible to image the entire temporal lobe of the brain without tilting the animal sample.

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Rotation

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Pan

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Tilt

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Easy Removal of Fluorescent Filter

The fluorescent filter is held in a removable filter cube. the fluorescent filter can be changed by replacing the filter cube.

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Multifunction: Used As a Fluorescence Beam Splitter

Normally, an excitation light source is connected to the left port of the THT main unit and used as a coaxial epi-illuminator.

In addition, it can be used as a two-wavelength fluorescence splitting device. In this case, the same lens and camera as the upper port are connected to the left port, and two-wavelength fluorescence simultaneous measurement using two cameras is performed.

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Inverted type is also possible

An inverted fluorescence mesoscope has been manufactured. The space on the sample stage becomes freer, so we expect that the degree of freedom in manipulator installation and electrode operation will increase. It can also be used with an upright fluorescent microscope. Please feel free to ask us for microscope bases and sample stages to suit the purpose of the experiment.

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• Epi illumination/single wavelength imaging

• Epi-illumination/dual wavelength imaging (upright)

• Epi-illumination/dual wavelength imaging (45 degrees)
• Epi-illumination/dual wavelength imaging (90 degrees)

• Epi-illumination
  Single wavelength imaging
  Brainvision XY stage included

• Boom-arm stand

• Extension arm

• Olympus BX51WI base
  includes manual XY table from Luigs&Neumann, Germany

• Horizontal imaging for Langendorff perfused heart

• Epi illumination
  dual-wavelength imaging

• Triple-wavelength imaging

• Side illumination
  dual-wavelength imaging

• Dual-wavelength imaging, lower position

• Horizontal imaging for Langendorff perfused heart

Name	Fluorescence Mesoscope THT Mesoscope
Optical System	Tandem lens optical system used for camera only
Focusing device	Manual coarse/fine focus drive or Motorized coarse/fine focus drive (incl. dial type controller)
Microscope carrier	Objective nosepiece (two M65 ports), or tilting mount
Filter Cube	Removable One 50mm diameter filter can be installed on the top and side Dichroic mirror mount with 2-axis tilt adjustment mechanism
Illumination Light for Excitation	Coaxial epi-illumination or side illumination
Lens port	Top: 1, Side: 1, Bottom: 1
Objective lens	PLAN 0.3x PLAN APO 0.63x PLAN APO 1x PLAN APO 1.6x PLAN APO 2x PLAN APO 5x
Projection lens	PLAN APO 0.63x PLAN APO 1x PLAN APO 1.6x PLAN APO 2x 135mm/2.0 lens (w/ aperture and focus adjustment function) 85mm/1.4 lens (w/ aperture and focus adjustment function) 50mm/0.95 lens(w/ aperture and focus adjustment function)

Approximate magnification and field size for N256 camera and THT Mesoscope

Reference papers for neuronal imaging

Neuronal Imaging

Cortical State Fluctuations during Sensory Decision Making.

Elina A.K. Jacobs, Nicholas A. Steinmetz, Andrew J. Peters, Matteo Carandini, Kenneth D. Harris

Curr Biol . 2020 Dec 21;30(24):4944-4955.e7.

Functional Differentiation of Mouse Visual Cortical Areas Depends upon Early Binocular Experience.

Kirstie J. Salinas, Carey Y. L. Huh, Jack H. Zeitoun and Sunil P. Gandhi

Journal of Neuroscience 17 February 2021, 41 (7) 1470-1488

Cellular and Widefield Imaging of Sound Frequency Organization in Primary and Higher Order Fields of the Mouse Auditory Cortex.

Sandra Romero, Ariel E Hight, Kameron K Clayton, Jennifer Resnik, Ross S Williamson, Kenneth E Hancock, Daniel B Polley

Cerebral Cortex, Volume 30, Issue 3, March 2020, Pages 1603-1622

Cardiac Imaging

Whole-heart multiparametric optical imaging reveals sex-dependent heterogeneity in cAMP signaling and repolarization kinetics.

Jessica L Caldwell, I-Ju Lee, Lena Ngo, Lianguo Wang, Sherif Bahriz, Bing Xu, Donald M Bers, Manuel F Navedo, Julie Bossuyt, Yang K Xiang, Crystal M Ripplinger

Sci Adv. 2023 Jan 20;9(3):eadd5799.

Optional parts

Lens adaptor

• CDAD-HL Lens Adaptor (Code No.: CDAD-HL)

Iris

• Imaging lens base with iris (Code No.: SM3D50-PRJM65)

Light guide arm

• Light Guide Holder
• Light Guide Arm