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Cardiac Imaging Sample Data

High-Speed Calcium Imaging of Isolated Mouse Atrial Cardiomyocytes

Sample
Freshly isolated mouse atrial cardiomyocytes. Cells were plated on glass cover slips coated with laminin and used on the same day as isolation.
Method
Cells were paced by electrical field applied through a perfusion solution. Imaging was performed on Nikon DIAPHOT 300 microscope with 20X lens and SPECTRA-X Lumencor light source.
Fluorescence Dye
Calcium sensitive dye (Fluo-4 AM)
Imaging System
MiCAM05-ULTIMA
Pixels
100x100 with 20X/NA1.0 lens
Frame Rate
500fps (2.0msec/frame)
Provided by
Dr. Roman Medvedev and Dr. Alexey Glukhov, Department of Medicine, University of Wisconsin-Madison

The Nervous Heart: Insights into Autonomic-Mediated Arrhythmias


Monolayers of neonatal mouse ventricular myocyte stained with voltage sensitive dye

Sample
Monolayer of neonatal mouse ventricular myocyte. Sample was scratched with a needle tip at three locations (visible on the fluorescence pictures) to induce conduction blocks and meandering of the wavefront.
Method
Sample is paced locally at different frequencies using a glass microelectrode positioned at the edge of the sample
Fluorescence Dye
Voltage sensitive dye (Di-8-Anepps)
Imaging System
MiCAM05-N256
Pixels
256x256
Frame Rate
1,000fps (1.0msec/frame)
Provided by
Dr. Jan Kucera and Dr. Ange Maguy, Department of Physiology, University of Bern

Cardiac Imaging Data Analysis using BV Workbench



High Speed Imaging of Atrial and Ventricular Action Potential


Animal
Mouse
Sample
Isolated heart
Dye
Voltage Sensitive Dye
Imaging System
MiCAM03-N256
Pixels
256x256
Frame Rate
1,000fps (1.0msec/frame)

Ventricular Fibrillation in Pig Heart



Animal
Pig
Sample
Isolated Heart
Dye
Voltage Sensitive Dye
Imaging System
MiCAM03-N256
Pixels
256x256
Frame Rate
1,818fps (0.55msec/frame)
Provided by
Dr. Jack M. Rogers, The University of Alabama at Birmingham

Ventricular Fibrillation in Pig Heart

Ventricular Fibrillation in Pig Heart


Imaging System
MiCAM02-HR
Provided by
Dr. Mihaela Pop, Sunnybrook Research Institute, Dept Medical Biophysics, University of Toronto

Calcium Imaging in Cultured Cardiomyocytes (Fluo-4)

Calcium Imaging in Cultured Cardiomyocytes (Fluo-4)


The preparation was cultured rat cardiomyocytes stained with Fluo-4 and activity was recorded using MiCAM01 (old model) in 2002. The current model, MiCAM02 can significantly improve the data in terms of spatial resolution, sensitivity and S/N ratio.


Simultaneous Calcium and Voltage Imaging in Human Ventricle

Simultaneous calcium and voltage imaging in human ventricle (MiCAM ULTIMA-L dual camera)


Simultaneous calcium and voltage imaging in human ventricle with MiCAM ULTIMA-L dual camera system. Figure shows (left to right): photo of transnural wedge human heart preparation, single pixel recordings of Vm and Ca, maps of action potential duration and calcium transient duration at 80% amplitude.

Provided by
Dr.Qing Lou and Dr.Igor Efimov, Washington University in St.Louis


Ventricular Fibrillation in Guinea Pig Heart (MiCAM ULTIMA-L)

Ventricular fibrillation (VF) in guinea pig heart. VF was induced by burst stimulation (35 ms interval, 100 pulses) and field of view is exactly 1x1 cm (1:1 magnification) from anterior region of heart. The first derivative of action potential was calculated to detect wave fronts as shown in this trace. The animation of action potential propagation clearly shows details of the direction and the curvature of the wave front. The heart was retrogradely perfused at 70 mmHg perfusion pressure and 20 ul of di-4 ANEPPS stock solution (1 mg / 1 mL DMSO).

Provided by
Dr.Bum-Rak Choi, Cardiovascular Research Center, Rhode Island Hospital and Brown Medical School

Cultured Neonatal Rat Ventricular Cardiomyocytes (MiCAM ULTIMA-L)

Cultured Neonatal Rat Ventricular Cardiomyocytes (MiCAM ULTIMA-L)


Sustained spiral wave reentry in a 3 day old monolayer disc of cultured neonatal rat ventricular cardiomyocytes (frequency ~ 5 Hz; diameter of the preparation = 8 mm). The preparation was stained with the voltage sensitive dye di-8-ANEPPS and activity was recorded at 1,000 frames/second using a custom-made tandem lens macroscope and an Ultima-L camera system.

Provided by
Dr.Stephan Rohr, University of Bern

Isolated  Guinea Pig Heart (MiCAM ULTIMA)

An action potential propagation in guinea pig heart. The heart was Langendorff perfuse and stained with PGH1. The anterior surface of heart was focused on 100x100 CMOS chip with 0.15x0.15 mm2 spatial resolution. The heart was stimulated from the left side of view and action potential propagation was recorded at 10,000 frames/second. The first derivative of action potential was calculated to detect wave fronts as shown in this trace. The animation of action potential propagation clearly shows details of the direction and the curvature of the wave front.

Provided by
Dr.Guy Salama and Dr.Bum-Rak Choi, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine

Whole Heart Imaging with Low Magnification (MiCAM ULTIMA-L)

An action potential propagation in isolated rat heart. The heart was Langendorff perfuse and stained with Di-4-ANEPPS and recorded at 2,000 frames/second using MiCAM ULTIMA-L and SciMedia's custom-made tandem lens microscope.

Provided by
Dr.Yu-Shien Ko, Chang Gung Memorial Hospital

Whole Heart Imaging with High Magnification (MiCAM ULTIMA-L)

An action potential propagation in isolated mouse heart. The heart was Langendorff perfuse and stained with Di-4-ANEPPS and recorded at 10,000 frames/second using MiCAM ULTIMA-L and SciMedia's custom-made tandem lens microscope.

Provided by
Dr.Yeh and Dr.Lai, Chang Gung Memorial Hospital

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Cardiac Imaging