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Neuronal Imaging Sample Data

Rat In Vivo Imaging using Voltage-Sensitive Dye (MiCAM02-HR)

Rat In Vivo Imaging using Voltage-Sensitive Dye (MiCAM02-HR)


Animal
Rat
Sample
In Vivo (Insular Cortex)
Method
Electrical stimulation (a train of 5 stimuli with 20msec interval)
Dye
RH1691
Sampling Rate
4.0ms/frame
Averaging
15 times
Provided by
Dr.Satoshi Fujita and Dr.Masayuki Kobayashi
Nihon University School of Dentistry, Japan
Reference
Fujita S, Adachi K, Koshikawa N, Kobayashi M.
Spatiotemporal dynamics of excitation in rat insular cortex: intrinsic corticocortical circuit regulates caudal-rostro excitatory propagation from the insular to frontal cortex.
Neuroscience. 2009 Oct 1. [Epub ahead of print]

Mouse In Vivo Imaging using Voltage-Sensitive Dye (MiCAM ULTIMA)

Mouse In Vivo Imaging using Voltage-Sensitive Dye


Voltage-sensitive dye imaging of in-vivo mouse with MiCAM ULTIMA.
This data was obtained from a 2 month-old mouse that was under urethane anaesthesia (1.75 g/kg). A heating pad was used to maintain the measured body temperature of the mouse at 37°C. Two electrodes were inserted under the skin of the forearms to receive the electrocardiogram signal which was used to trigger the MiCAM ULTIMA acquisition.

Animal
Mouse
Sample
In Vivo (Somatosensory Barrel Cortex)
Method
Piezo Whisker Flick Stimulation
Dye
RH1691
Sampling Rate
2.0ms/frame
Averaging
10 times
Provided by
Dr.Isabelle Ferezou and Dr.Carl C.H. Petersen
Brain Mind Institute, EPFL, Switzerland
Reference
Visualizing the Cortical Representation of Whisker Touch: Voltage-Sensitive Dye Imaging in Freely Moving Mice.
Neuron. 2006 May 18;50(4):617-29.

Rat Cortex Slice (MiCAM02-HR)

Membrane potential imaging of rat cortex slice with MiCAM02-HR camera. Temporal resolution is 384x256 pixels and frame rate is 7.0msec/frame.

Animal
Rat
Sample
Cortex Slice
Method
Electrical stimulation
Dye
Di-4-ANEPPS (D-1199)
Sampling Rate
7.0ms/frame
Averaging
4 times
Provided by
Dr.Takashi Tominaga and Dr.Michinori Ichikawa, Brain Science Institute, RIKEN

Isolated Guinea Pig Brain (MiCAM ULTIMA-L)

Action potential propagation in isolated guinea pig brain stained with voltage-sensitive dye.

Animal
Guinea Pig
Sample
Isolated Brain
Method
Electrical stimulation
Dye
Di-4-ANEPPS (D-1199)
Sampling Rate
1.0ms/frame (1,000Hz)
Averaging
8 times

Rat Slice Imaging at 10KHz (MiCAM ULTIMA-L)

Action potential propagation was recorded at 0.1msec/frame. 10kHz recording allows us to identify presynaptic activity and postsynaptic activity separately.

Animal
Rat
Sample
Hippocampus Slice
Method
Electrical stimulation
Dye
Di-4-ANEPPS (D-1199)
Sampling Rate
0.1ms/frame (10,000Hz)
Averaging
4 times
Provided by
Dr.Takashi Tominaga and Dr.Michinori Ichikawa, Brain Science Institute, RIKEN

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Neuronal Imaging